Home · About CCP4 · CCP4 Projects · Downloads · Documentation · Courses · Developers · CCP4 people · WG1/WG2 · Privacy. Other MR examples can be found at the end of this tutorial, and at: When this tutorial is obtained as part of the CCP4 distribution, $MR_TUTORIAL. Previously Lecture Notes and Tutorial Material. 1、 Lecture Notes and Tutorial Material. iMosflm training · Mosflm examples. 2、 Lecture Notes and .
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The graphs in Acentric Moments of It is not unusual for this to be higher for two images than for a single image, because the crystal orientation may have changed between collecting the two images. It is considered good practice to add in all other reflections appropriate to the spacegroup and resolution, even if there is no data for them “completing the dataset” and to add a column of free-R flags at tutoriall stage to the master dataset.
See how sensitive the autoindexing is to the main beam values. If you have collected data from one crystal xtal1 using the phi range to 10, and now tutoral to collect data from xtal2 and want to know what phi range to collect to maximise completeness, do the following:. Cc;4 this stage, we can have a look at the anomalous Patterson for these data.
CCP4 Molecular Graphics – Tutorial Contents
As an exercise, we are going to ttuorial MR straightaway. Using Phased RF and TF Positioning the large and small domains of sucrose-phosphatase using conventional Patterson search and search in electron density map, respectively. The information that is displayed comes from the header of the MTZ file. If it is bigger than Normally the integration would be done as a background job it is times faster, because tutoial is a big overhead associated with displaying the images in the GUI.
To do this, in the Temperature Factor Analysis task window, click on the View button in the line where the PDB file was selected – this will display a CCP4i fileviewer window with the contents of toxd.
Crank pipeline for experimental phasing Automated tutlrial solution using Crank pipeline and inflection point data. The “Output” panel displays information on mouse position click left mouse button or on particular reflections if a predicted pattern is displayed click left mouse on prediction box.
As established abovebatch number 74 is a rather serious outlier. The program will then work out the best two 15 degree segments. This example will find two 20 degree segments that give maximum completeness of course, the multplicity will suffer!
If you have done more than one integration run during your MOSFLM session, you tutoril find multiple entries of the tables. This ratio is tabulated in Standard deviation v.
See if there is anything odd about the spot. We need tutoril supply the spacegroup and cell dimensions, since these are not included in the input CNS file.
The default values are 0. At the end of refinement, the distance should be the same for all images, typically to within 0. The contrast indicates that this is probably a correct solution, but this should now be checked! This protein has a Hg derivative.
CCP4 Tutorial: Session 2 – Data Processing and Reduction
Molrep can make use of the target sequence to improve the search model. The search model has a moderately low sequence identity with the target and therefore the majority of the side chains are incorrect.
You also need to understand how information is arranged in an MTZ file. In fact, we able to improve on this solution. We will return to Chainsaw later before running Phaser. Fractional bias may show an indication of “Partial bias”. Read what is in the small window because you have the option of changing both the beam position and the position and size of the backstop; you only want to do the former in this case!
Some of the residues have Bfactors of 0. If you do exit, follow the instructions at the end of this section on how to restart from saved parameters. For these data, the true direct beam position is within 0. Investigate this in the exercise below. This should fill in the bulk of the interface window.