Confirm manual entries with ENTER. Exit the parameter level at any time by repeatedly pressing the. “Parameter” key. BioPhotometer – Short instructions. Get Eppendorf BioPhotometer Manual. Get all Eppendorf manuals!. Get Eppendorf BioPhotometer plus Instruction Manual. Get all Eppendorf manuals!.
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Absorbance, Factor, Standard and Warburg formula.
The result is calculated taking into account the sample dilution entered. The device may only be operated by trained specialist staff. The exact measurement wavelength for the BioPhotometer biopohtometer is nm. Tell us what’s missing. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.
Blophotometer B R A D. Recalibrate or use the calibration stored. Page 49 Page 50 Page 51 In touch with life Your local distributor: Service product portfolio may vary according to country.
Eppendorf BioPhotometer User Manual | 53 pages
The scattering of the measured values around the calculated calibration line or curve is very large see Section 12, “Calculation”. In practice, nucleic acids are purified with the help of commercially available kits which allow for the separation of most cellular components. Circular keys Start standard measurement. However, the presence of proteins or other organic components in the eluate may not be entirely eliminated.
It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. In addition, all important raw data pertaining to the DNA sample are displayed in table format.
Caution when using aggressive chemicals. Sample dilution and optical light path of the cuvette See Section Users are guided through every step of the assay with biophoyometer, simple on-screen instructions.
Connect the printer cable to the serial printer port 4 of the photometer and tighten the locking screws. Easy closing for easy handling. Aggressive chemicals biophotomeer damage both the device and its accessories. Front and rear view Fig. Similar dimensions as classic cuvettes. The method was calculated using the Warburg formula.
In touch with life Your local distributor: Safety socket — Insert the mains plug of the device into the safety socket. Parameter Entries Explanation Std.
Eppendorf BioPhotometer : Manual
BioPhotometer plus — Operating manual 5 Operation 5. BioPhotometer plus — Operating manual 8 Troubleshooting 8. Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample.
Page 9 BioPhotometer plus — Operating manual 2 Product description The BioPhotometer plus converts the measured absorbance values into concentration results. In general, the purity of a DNA solution is easily defined using purity ratios.
When selecting the cuvettes observe the respective instructions see Cuvettes on biophotomwter Operating manual A list of general device functions will open. The sample number is counted separately for each method and is reset to “1” when the biphotometer changes.
Nucleic acid purification is a major application in molecular laboratories. Don’t see a manual you are looking for? Blank measurements remain stored until the date changes For further details, please refer to “Measuring nucleic acids”.
Tell us what’s missing. Box Westbury, N. It is not provided for protein methods. Measurements with suspensions of E. Starting with an ergonomic design of the product itself e. For connecting to a network printer or emailing data outputs directly off instrument.